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yurJ [2019-04-24 13:42:00]
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yurJ [2019-04-24 13:42:00]

aminosugar ABC transporter (ATP-binding protein)
locus
BSU32550
pI
6.07
mw
41.19 kDa
protein length
367 aa Sequence Blast
gene length
1104 bp Sequence Blast
function
uptake of sugar amines
product
aminosugar ABC transporter (ATP-binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.1|Uptake of carbon sources]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.17|Utilization of amino sugars]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.3|Utilization of amino sugars]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    3,345,013 3,346,116

    The protein

    Paralogous protein(s)

  • [protein|434F6C546F783503A82D390FD238F05025FC1802|MsmX]

    • Modification

  • phosphorylated on Ser-3 [Pubmed|20509597]

    • Structure

  • [PDB|1G29] (from Thermococcus litoralis, 50% identity) [pubmed|11080142]

    • [SW|Localization]

  • membrane associated (via [protein|1F245030D0C81DC96FC0642199CA00579CF46D06|FrlM]-[protein|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|FrlN]) [Pubmed|10092453,18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|3B9331D6755B8253944AB33521BBF2B3EBA4CAEB|yurJ]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
    • view in new tab

    genes
    [gene|C87E74FB67B53DD1C68916AA121388C60CE66E92|frlB]-[gene|9E3FF9D0B33D7EFEA3B61FF863BB46CC6DFD4F9B|frlO]-[gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]-[gene|1F245030D0C81DC96FC0642199CA00579CF46D06|frlM]-[gene|C3823AFED3E5C3D1EA04C38D861239468C9E6349|frlD]-[gene|3B9331D6755B8253944AB33521BBF2B3EBA4CAEB|yurJ]
    description
    [Pubmed|23175651,21347729,22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|22383849], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [pubmed|12618455], [pubmed|18083814], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|412A1DBBE7F87765DEBEEAAE02A047636A354D5E|FrlR]: repression, [Pubmed|21398478], in [regulon|412A1DBBE7F87765DEBEEAAE02A047636A354D5E|FrlR regulon]
  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]

    • regulation

  • '' [protein|C87E74FB67B53DD1C68916AA121388C60CE66E92|FrlB]'': repressed during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]) [Pubmed|12618455]
  • induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]

    • additional information

  • the [gene|C87E74FB67B53DD1C68916AA121388C60CE66E92|frlB]-[gene|9E3FF9D0B33D7EFEA3B61FF863BB46CC6DFD4F9B|frlO]-[gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]-[gene|1F245030D0C81DC96FC0642199CA00579CF46D06|frlM]-[gene|C3823AFED3E5C3D1EA04C38D861239468C9E6349|frlD]-[gene|3B9331D6755B8253944AB33521BBF2B3EBA4CAEB|yurJ] operon is strongly downregulated in a [gene|search|cshA ]mutant [Pubmed|23175651]
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    Biological materials

    Mutant

  • MGNA-A556 (yurJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/556 NBRP B. subtilis, Japan]
  • BKE32550 ([gene|3B9331D6755B8253944AB33521BBF2B3EBA4CAEB|yurJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGAAAAGTCCCCCTAT, downstream forward: _UP4_TAGAAAAAGGTGCTCCTCAA
  • BKK32550 ([gene|3B9331D6755B8253944AB33521BBF2B3EBA4CAEB|yurJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGAAAAGTCCCCCTAT, downstream forward: _UP4_TAGAAAAAGGTGCTCCTCAA

    • References

  • 12618455,10092453,18763711,20509597,11080142